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Evaluating Protein Quality

CalorieKing.com Staff


 

Not all proteins are equal. In order to assess how valuable a source of protein is, scientists have developed the Protein Efficiency Ration (PER) and the Protein Digestibility Corrected Amino Acid Score (PDCAAS).

To understand more about how these methods work, read on.


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Protein Efficiency Ratio

The traditional method for evaluating protein quality has been the protein efficiency ratio (PER) which has been in use since 1919. PER is a measure of the weight gain of a growing animal divided by the amount of protein that the animal consumes.

The PER is based on the growth of rats, in response to a given amount of protein. However, rats have different amino acids requirements than we do. In particular, their replacement for methionine is thought to be 50 percent higher. Consequently, the PER undervalues the quality of legume proteins.

How to Calculate PER

PER = weight gain (grams) divided by protein intake (grams)


The Protein Digestibility Corrected Amino Acid Score

Recently, the Protein Digestibility Corrected Amino Acid Score (PDCAAS) was introduced as a more accurate way to evaluate protein quality for children and adults. The PDCAAS has now been adopted as the official method by the World Health Organization, the U.S. Food and Drug Administration and the U.S. Department of Agriculture.

The PDCAAS is the amino acid score (amino acid pattern of a protein relative to amino acid needs) with a correction factor for digestibility. The PDCAAS uses human, not rat, amino acid requirements to calculate the amino acid score. Specifically, the amino acid requirements used are those established by the World Health Organization (WHO) for two- to five-year-old children. Those proteins that meet the WHO guidelines are a given a rating of 1.

Soy protein contains enough of all the essential amino acids to meet biological requirements when consumed at the recommended level of protein intake.

The PDCAAS for soy protein isolate is 1.0, which makes soy equivalent to animal proteins in quality and higher than other plant proteins.

How to Calculate PDCASS

  • Analyze for proximate nitrogen (N) composition
  • Calculate protein content (N*6.25 or specific AOAC conversion factor)
  • Analyze for essential amino acid (Eaa) profile
  • Determine the amino acid score (uncorrected). The equation is:

    Mg of Eaa in 1g of test protein divided by mg of Eaa in 1g of reference protein

  • Analyze for digestibility
  • Calculate the PDCAAS:

    PDCASS = Lowest uncorrected amino acid score * protein digestibility

Recommended protein intake

A 4 oz serving of cheese provides around half your recommended daily protein intake

On average, Americans eat too much protein. The current recommendation is that protein makes up around 15-20 percent of your total calories for the day. Protein has four calories per gram, so for a 2000 calorie-a-day diet you could eat 75-100 grams of protein. However, most of us consume more than that each day. Eating too much protein can be bad for your body.

Growing children need about one gram of protein for every two pounds of body weight. For example, a 56 pound child needs about 28 grams of protein a day to sustain her growth.


Mini protein counter

Adapted from The Doctor's Pocket Calorie, Fat and Carbohydrate Counter. For more information, see the book or the CalorieKing.com online food database.

Animal protein foods:

Protein (g)

Meat, cooked, lean, 3 oz

23g

Chicken, leg/thigh, lean, 3 oz

24g

Milk (whole or skim), 1 cup, 8 oz

8g

Yogurt, average, 6 oz

8g

Cheese, 4 oz piece

28g

Cottage Cheese, ½ cup

13g

Egg, 1 large

6g

Ice cream, regular vanilla, ½ cup

2.5g

McDonald's, Big Mac

24g

McDonald's, Cheeseburger

15g

Burger King Whopper

29g

Pizza Hut, Pan pizza, medium, two slices

26g

Yeast, dry, 1 Tbsp

5g

Plant protein foods:

 

Soy Beans, cooked, ½ cup, 3 oz

14g

Lentils/Dry Peas, cooked, ½ cup, 3½ oz

9g

Baked Beans, ½ cup, 4½ oz

7g

Peanuts, dry roasted, 40 nuts, 1 oz

6g

Soy milk (fortified), average, 1 cup

7g

Tofu, raw, firm, ½ cup

10g

Tempeh, ½ cup, 3 oz

16g

Oatmeal, regular, non-fortified, 1 cup

6g

Wheat germ, 1 Tbsp

4g

Cheerios, regular, 1 cup, 1 oz

3g

All Bran, ½ cup

4g

Bread, average, 4 slices, 4 oz

8g

Potato, cooked, 1 medium, with skin, 5 oz

3.3g

Rice, brown/white, average, 1 cup, cooked, 6½ oz

5g

Spaghetti, Pasta, cooked, enriched, 1 cup, 4½ oz

7g

Vegetables, average, 1 cup

4g

Fruit, average, 1 med/2 small

1g

Sugar, Soda, Wine Spirits

0g

Beer, 12 fl oz

1g

 


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Last updated: February 14th, 2006

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